Table 2:

Lifetime Measurements on IHF-DNA Complexes

Construct	〈τ1〉 (ns)	〈E1〉	α 1	〈τ2〉 (ns)	〈E2〉	α 2	$\Delta G/k_{B}T=-\text{ln}\left(\frac{{\alpha }_{\text{1}}}{{\alpha }_{\text{1}}}\right)$
IHF-H’ (design I)	1.52 ± 0.06a (1.41 ± 0.03)b	0.65 ± 0.03 (0.67 ± 0.02)	78 ± 3% (66 ± 2%)	3.96 ± 0.01 (4.13 ± 0.02)	0.085 ± 0.002 (0.043 ± 0.001)	22 ± 3% (34 ± 2%)	−1.3 ± 0.2 (−0.66 ± 0.04)
IHF-H’ (design II)	2.06 ± 0.02	0.51 ± 0.01	48 ± 3%	3.00 ± 0.01	0.29 ± 0.01	52 ± 3%	0.090 ± 0.008
IHF-H1 (design I)	2.14 ± 0.14	0.50 ± 0.03	32 ± 1%	3.71 ± 0.12	0.14 ± 0.01	68 ± 1%	0.75 ± 0.03
IHF-H’_nAt (design I)	1.87 ± 0.01	0.57 ± 0.01	33 ± 1%	3.99 ± 0.04	0.077 ± 0.001	67 ± 1%	0.70 ± 0.02
IHF-H1_CTloop (design I)	1.96± 0.08	0.55 ± 0.02	43 ± 3%	3.22 ± 0.16	0.25 ± 0.01	57 ± 3%	0.32± 0.03
IHF-H’44A (design I)	1.66 ± 0.04	0.62 ± 0.02	32 ± 1%	3.99 ± 0.01	0.076 ± 0.001	68 ± 1%	075 ± 0.03

^aMeasurements were done in 100 mM KCl

^bValues in parenthesis are for measurements done in 200 mM KCl