Table 2.

Sequences of real-time PCR primers

Gene	Primer sequence (5'-3')	Annealing temperature
HSP 70	F—TTCGTGGACAGAAGCCACAG R—TTGCTAGGATCTCCACCCGA	55
HSP 90	F—GTCGAAAAGGTGGTTGTGTCG R—TTTGCTGTCCAGCCGTATGT	55
IL 1 β	F—CCAAAGAGGGACATGGAGAA R—GGGCTTTTGTTCTGCTTGAG	55.7
TNF α	F—CGTCGCCCACGTTGTAGCCAAT R—GCCCATCTGTCGGCACCACC	55.7
MUC 2	F—AACCAGAAGCTGGTCCTGAA R—TGTCAGCCATCGTAGGAAAT	55
PEPT 1	F—CAGACTTCGACCACAACGGA R—TTATCCCGCCAGTACCCAGA	55
SGLT 1	F—GTGCAGTCAGCACAAAGTGG R—CCCGGTTCCATAGGCAAACT	55
FUT 2	F—CGAGTGGATTGGGATCGAGG R—AAGAAGAATGGGGAGCCGAG	55
Claudin 4	F—CTCTCGGACACCTTCCCAAG R—GCAGTGGGGAAGGTCAAAGG	55
Occludin	F—CTACTCGTCCAACGGGAAAG R—ACGCCTCCAAGTTACCACTG	61
GAPDH	F—GTTTGTGATGGGCGTGAAC R—ATGGACCGTGGTCATGAGT	55