Table 6.

Effect of carbohydrase supplementation and environmental temperature on jejunal gene expression of pigs fed wheat-based diets¹

	Carbohydrase level			Temperature		P-value
	CON	1X	2X	TN	HS	Enz	Temp
HSP70	1.7 ± 0.34	1.0 ± 0.36	1.4 ± 0.36	1.3 ± 0.31	1.4 ± 0.31	0.154	0.616
HSP90	1.4 ± 0.21	1.0 ± 0.21	1.2 ± 0.21	1.1 ± 0.19	1.3 ± 0.19	0.400	0.294
MUC2	1.2 ± 0.14	0.9 ± 0.17	1.2 ± 0.16	1.2 ± 0.12	1.0 ± 0.13	0.604	0.479
IL1β	1.3 ± 0.14	1.3 ± 0.16	1.4 ± 0.16	1.0 ± 0.13	1.6 ± 0.14	0.818	0.0003
TNFα	1.4 ± 0.19	1.4 ± 0.2	1.4 ± 0.2	1.2 ± 0.16	1.5 ± 0.15	0.981	0.189
PEPT1	0.7 ± 0.15	0.8 ± 0.13	0.7 ± 0.13	0.6 ± 0.11	0.8 ± 0.12	0.585	0.157
SGLT1	0.7 ± 0.09	0.6 ± 0.09	0.6 ± 0.09	0.6 ± 0.08	0.6 ± 0.08	0.641	0.833
FUT2	1.7 ± 0.5	1.9 ± 0.52	1.7 ± 0.5	1.7 ± 0.44	1.9 ± 0.41	0.924	0.661
Claudin 4	1.5 ± 0.24	1.6 ± 0.22	1.4 ± 0.26	1.6 ± 0.2	1.4 ± 0.2	0.831	0.713
Occludin	1.6 ± 0.18	1.4 ± 0.18	1.8 ± 0.18	1.6 ± 0.15	1.6 ± 0.14	0.441	0.972

¹Data are means of 16 replicates per treatment ± standard error in carbohydrase level and 24 replicates per treatment ± standard error among temperature. Means with different superscript are different (P < 0.05). CON, control; 1X, 50 g/tonne carbohydrase admixture; 2X, 100 g/tonne of carbohydrase admixture. HSP70, heat shock protein 70; HSP90, heat shock protein 90; MUC2, mucin 2; IL1β, interleukin 1β; TNFα, tumor necrosis factor α; PEPT1, peptide transporter type 1; SGLT1, sodium dependent glucose cotransporter type 1; FUT2, fucosyltransferase type 2. TN, thermoneutral; HS, heat stress; Temp, temperature; Enz, carbohydrase treatment.