Table 1.
Characteristics of synthesized peptides
| Code | Sequence | Mol. Mass, g/mol | MALDI-TOF, m/z | Purity HPLC, % |
|---|---|---|---|---|
| SPB1 | IEEQAKTFLDKFNHEAEDLFYQS-NH2 (control) | 2802.01 | 2801.4 | 97.6 |
| h1 | IEEQAKTFLDKFNHEAEDLFYk | 2716.0 | 2715.4 | 97.26 |
| h1-D-Cys | cEEQAKTFLDKFNHEAEDLFYk | 2705.95 | 2705.3 | 96.8 |
| h1-Mpa | IEEQAKTFLDK(Mpa)FNHQAEDLFYk | 2802.4 | 2802.5 | 96.3 |
| h2 | DKWSAFLKEQSTIAQNleYPLQECI | 2712.1 | 2711.4 | 95.9 |
| h2-Cys | DKWSAFLKECSTIAQIYPLQEI-OH | 2584.0 | 2583.1 | 96.4 |
| Х1 |
|
5412.9 | 5413.9 2711.5* 2705.4* | 95.0 |
| X2 |
|
5383.9 | 5382.8 2583.4* 2802.5* | 96.2 |
Modifications are shown in bold; lowercase letters designate D-amino acids; *m/z correspond to the molecular weights of the daughter peptides constituting the disulfide; Mpa—3-mercaptopropionic acid.