FIG. 2.

Differential expression of clones isolated from the screen by virtual Northern blotting (A) and RT-PCR (B). Rpl19 probe was used as a control for both the virtual Northern blotting and RT-PCR. Both Sgce and Zac1 are expressed only in the AG and WT MEFs by both virtual Northern blotting and RT-PCR. Other genes such as Gas and Igfbp5 are biallelically expressed. The maternally expressed H19 was analyzed by RT-PCR as a control for AG MEFs not expressing maternally expressed imprinted genes (abbreviations: M, markers; N, WT MEFs; P, PG MEFs; A, AG MEF cDNA).