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The EMSAs were used to determine the interaction between promoter-emhABC and RstA, phosphorylated-RstA (A, B), RstAD52A or phosphorylated-RstAD52A (C, D). The binding between promoter-mexC and RstA, phosphorylated-RstA, RstAD52A, or phosphorylated-RstAD52A was determined with the same methodology. The concentrations of protein were labeled at the top of each lane. The protein phosphorylation state was indicated with + or –, respectively.
