TABLE 3.

Protein functions inferred by structural similarity and confirmed through manual structural analysis^a

Rv no.	PDB ID	AA %	Inferred EC	Updated EC	Change	Recommended product name	Product name (Mycobrowser)
Rv0036c	2nsg	14%	5.2.1.-	None	Reduced specificity	Putative thiol-dependent DinB-like metalloenzyme	Conserved protein
Rv0738	2nsg	17%	5.2.1.-	None	Reduced specificity	Putative thiol-dependent DinB-like metalloenzyme	Conserved protein
Rv1632c	2p12	59%	3.6.1.-	3.6.1.-	Verified	Putative cytidylyl-2-hydroxypropylphosphonate hydrolase	Hypothetical protein
Rv1727	2nsg	12%	5.2.1.-	None	Reduced specificity	Putative thiol-dependent DinB-like metalloenzyme	Conserved hypothetical protein
Rv1734c	3l60	26%	1.2.4.-	2.3.-.-	Changed function	Putative acyltransferase	Conserved hypothetical protein
Rv1775	3hwp	29%	3.-.-.-	3.7.1.-	Increased specificity	Putative 3-oxo-carboxylic acid hydrolase	Conserved hypothetical protein
Rv2036	2nsg	15%	5.2.1.-	None	Reduced specificity	Putative thiol-dependent DinB-like metalloenzyme	Conserved hypothetical protein
Rv2968c	3kp9	21%	1.1.4.-	1.17.4.-	Nomenclature change	Putative vitamin K epoxide reductase	Probable conserved integral membrane protein
Rv3224B	1dbx	38%	3.1.1.-	None	Reduced specificity	Putative trans-editing enzyme	Conserved hypothetical protein

^aProteins with functions transferred from top PDB matches to their tertiary structural models subsequently verified through manually inspecting functionally essential protein features. Proteins shown lacked functional annotation output by HHpred corroborating the function from the structure-to-function annotation pipeline. Most had the same PDB template most closely matching the I-TASSER modeled structure among the top hits in HHpred but without functional information output and typically among dozens of other hits in PDB with similarly high match confidence uncorroborated by product function from HHpred.