Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

[Preprint]. 2021 Oct 29:rs.3.rs-1031824. [Version 1] doi: 10.21203/rs.3.rs-1031824/v1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is licensed under a Creative Commons Attribution 4.0 International License, which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.

License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License

PMC Copyright notice

Microglia and neurons contribute to neuroinflammation in the hippocampi of viral-infected hamsters and COVID-19 patients, a Representative image of IBA1 in the hamster hippocampus at naïve, 2, 3, 4, 5, 8, and 14 dpi, showing staining for IBA1 (red) and DAPI (blue) at 20x and 63x and quantified for percent IBA1+ area. b Immunostaining for IL-1β and IBA1 in the hamster hippocampus at naïve, 2, 3, 4, 5, 8, and 14 dpi, presented as microscopy with IBA1 (red), IL-1β (green) and DAPI (blue) and percent IL-1β+ area and IL-1β+IBA1+ area, normalized to total IL-1B+ area. c, e Representative image of IBA1 in control and COVID-19 patient hippocampi, showing staining for IBA1 (magenta) and DAPI (blue) at 20x and 63x and quantified for percent IBA1+ area. d, f Immunostaining for IL-1β and IBA1 in hippocampi of control and COVID-19 patients, presented as microscopy with IBA1 (magenta), IL-1β (green) and DAPI (blue) and percent IL-1β+ area and IL-1β+IBA1+ area, normalized to total IBA1+ ar-ea. g. Representative images of IBA1 in the human adult hippocampus with high magnification images single channel. Sections stained with DAPI (blue), IBA1 (green), and DCX (red) in non-COVID-19 control. Scale bar, 25 μm. h Representative images of GFAP in the human adult hippocampus with high magnification images single channel. Sections stained with DAPI (blue), GFAP (green), and DCX (red) in non-COVID-19 control. The arrow points to a single DCX+/GFAP− neuron in the subgranular zone. Scale bar, 25 μm. i. Immunostaining for IL-6 and NeuN in hippocampi of control and COVID-19 patients, presented as microscopy with NeuN (red), IL-6 (green) and DAPI (blue) and percent IL-6+ area and IL-16+NeuN+ area, normalized to total NeuN+ area. Data were pooled from at least two independent experiments. Scale bars, 20 μm (20x) or 10 μm (63x). Data represent the mean ± s.e.m. and were ana-lysed by two-way ANOVA or Student’s t-test.