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. 2021 Sep 25;38(3):331–337. doi: 10.5511/plantbiotechnology.21.0519a

Figure 2. Changes in medium Ca2+ concentration do not inhibit upregulation of VND7-downstream genes. Three-day-old T87/35S::VND7-VP16-GR cells were treated with dexamethasone (DEX) in medium containing the indicated concentration of Ca2+ and sampled 24 h later. Total RNA extracted from these cells was subjected to semi-quantitative RT-PCR analysis for VND7-downstream genes. As the control (C), cells without medium exchange were used. Normal culture medium contains 120 mg l−1 Ca2+. UBQ10 was tested as an internal control gene. The analysis was repeated three times as biological replicates.

Figure 2. Changes in medium Ca2+ concentration do not inhibit upregulation of VND7-downstream genes. Three-day-old T87/35S::VND7-VP16-GR cells were treated with dexamethasone (DEX) in medium containing the indicated concentration of Ca2+ and sampled 24 h later. Total RNA extracted from these cells was subjected to semi-quantitative RT-PCR analysis for VND7-downstream genes. As the control (C), cells without medium exchange were used. Normal culture medium contains 120 mg l−1 Ca2+. UBQ10 was tested as an internal control gene. The analysis was repeated three times as biological replicates.