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. 2021 Sep 25;38(3):297–304. doi: 10.5511/plantbiotechnology.21.0610a

Figure 1. Schematic representation of the T-DNA regions of plasmids pBYR2HS, pTKB2, and pTKB3. pBYR2HS is a previously produced vector (Yamamoto et al. 2018a). 35S-p×2, CaMV 35S promoter with double-enhanced element; AtADH5′, 5′-untranslated region (UTR) of Arabidopsis thaliana alcohol dehydrogenase gene; TMV Ω, 5′-leader sequence of the tobacco mosaic virus; HSPter, terminator of a heat shock protein gene; Ext3′, tobacco extensin gene 3′ element; 35Ster, terminator of CaMV 35S; NOSter, NOS terminator; LIR, long intergenic region of the bean yellow dwarf virus (BeYDV) genome; SIR, short intergenic region of the BeYDV genome; C1/C2, BeYDV ORFs C1 and C2 encoding for replication initiation protein (Rep) and RepA, respectively; LB and RB, the left and right borders of the T-DNA region, respectively; Nos-p and Nos-t, NOS promoter and terminator, respectively; p19, a gene-silencing suppressor gene from the tomato bushy stunt virus; GOI, gene of interest.

Figure 1. Schematic representation of the T-DNA regions of plasmids pBYR2HS, pTKB2, and pTKB3. pBYR2HS is a previously produced vector (Yamamoto et al. 2018a). 35S-p×2, CaMV 35S promoter with double-enhanced element; AtADH5′, 5′-untranslated region (UTR) of Arabidopsis thaliana alcohol dehydrogenase gene; TMV Ω, 5′-leader sequence of the tobacco mosaic virus; HSPter, terminator of a heat shock protein gene; Ext3′, tobacco extensin gene 3′ element; 35Ster, terminator of CaMV 35S; NOSter, NOS terminator; LIR, long intergenic region of the bean yellow dwarf virus (BeYDV) genome; SIR, short intergenic region of the BeYDV genome; C1/C2, BeYDV ORFs C1 and C2 encoding for replication initiation protein (Rep) and RepA, respectively; LB and RB, the left and right borders of the T-DNA region, respectively; Nos-p and Nos-t, NOS promoter and terminator, respectively; p19, a gene-silencing suppressor gene from the tomato bushy stunt virus; GOI, gene of interest.