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Figure 3. Necrosis of leaves is suppressed by foliar spraying of high concentrations of sodium ascorbate (AsA). After expressing either human cullin protein, hCul1, or F-box protein hFbxw7 in agroinfiltrated leaves, tissue necrosis (gray area) was observed (A), but not when leaves were sprayed with 200 mM AsA (B). AsA was applied by foliar spray (C).