. Author manuscript; available in PMC: 2021 Nov 2.

Published in final edited form as: Biochemistry. 2016 Apr 28;55(18):2601–2612. doi: 10.1021/acs.biochem.6b00226

Table 2.

Membrane binding affinities and cellular entry efficiencies of CPPs

CPP	Total Cellular Uptake (MFI^Rho, %)^a	Cytosolic Entry (MFI^NF, %)^a	Apparent Endosomal Escape (γ, %)^a	SUV1 EC₅₀ (μM)	SUV2 EC₅₀ (μM)	Cytosolic Delivery Efficiency (%)
1	100	100	100	713 ± 76	166 ± 12	20
2	38 ± 5	37 ± 3	98 ± 15	2220 ± 510	248 ± 18	7.5
4	32 ± 6	42 ± 4	132 ± 26	2060 ± 310	170 ± 12	8.5
7	77 ± 8	85 ± 5	110 ± 13	1300 ± 180	139 ± 10	17
9	152 ± 13	307 ± 15	202 ± 20	290 ± 60	34 ± 4	62
11	278 ± 18	247 ± 40	89 ± 15	169 ± 42	111 ± 10	50
12	149 ± 9	598 ± 61	402 ± 48	231 ± 43	16 ± 1	121
Tat	43 ± 3^b	9.9 ± 1.7^b	23 ± 4^b	NB	2580 ± 420	2.0^c
R ₉	49 ± 3	20 ± 2	40 ± 5	>5000	880 ± 130	4.0 (4.4^c)
5.3	122 ± 7	773 ± 85	634 ± 79	13.7 ± 3.4	7.8 ± 1.0	156 (50^c)

All values are relative to that of CPP 1 (100%).

Values from reference ⁴⁰.

Values determined by fluorescence correlation spectroscopy⁴². The data presented the mean ± S.D. of three independent experiments. NB, no binding.