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. 2021 Sep 9;8(Pt 6):878–895. doi: 10.1107/S2052252521008125

Figure 5.

Figure 5

Additional ligands. (a) CEF in the active site and the stacking site (dotted oval) located between subunits D and C. A DF o − mF c OMIT difference electron-density map in the active site is shown in green and that in the stacking site in gray–green (at a 2.5σ contour level). Substantial CEF density in the active site is shown in green. There is also electron density for Pi present due to averaging over all unit cells in the crystal. The stacking site is not occupied (gray CEFs molecule). (b) At 50 ms the maximum occupancy of CEF in the active center is reached. The stacking site is substantially occupied (blue CEF molecule). Important residues and distances are marked in Å. (c) The covalently bound trans-EN is present in subunit A of the static cryostructure of BlaC when soaked with SUB (blue; 2F o − F c map at a 1.5σ contour level).