Table 2. Parameters for the mix-and-inject experiments.
Concentrations of ceftriaxone (CEF) and sulbactam (SUB) are shown as were flowed through the outer capillary line of the mixing injectors. Time delays are achieved after mixing in a constriction as per Calvey et al. (2019 ▸).
Water | SUB | CEF | CEF | CEF | |
---|---|---|---|---|---|
Δt m (ms) | (10) | 66 | 5 | 10 | 50 |
Ligand concentration (mM) | — | 100 | 200 | 200 | 200 |
Ligand buffer | — | 0.8 M AP pH 4.6 | 0.8 M AP pH 4.6 | 0.8 M AP pH 4.6 | 0.8 M AP pH 4.6 |
Ligand flow (µl min−1) | 74.5 | 54.5 | 76.7 | 74.5 | 71.8 |
Crystal flow (µl min−1) | 5.5 | 11.6 | 3.3 | 5.5 | 8.2 |
Mixing injector capillary internal diameter (µm) | 50 | 75 | 50 | 50 | 75 |
Constriction length (mm) | 17.8 | 36.1 | 9.3 | 17.8 | 36.1 |
Timing uncertainty (ms) | — | 9.3 | 1.8 | 3.0 | 10.4 |
Experimental time to collect the data set (min) | 50 | 56 | 138 | 250 | 32 |