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Mitotic progression upon endogenous LIC1 depletion in WT and triple phosphodeficient LIC1 mutant stable U2OS cells. Time-lapse confocal videos of LIC1 siRNA–treated stable U2OS cell lines expressing MTAP-tagged WT LIC1 construct SST and triple site LIC1-CTD mutant AAA. Green represents YFP fluorescence from the MTAP tag. All videos show the duration from cell rounding to anaphase onset. Time stamps included. Scale bar = 10 µm. Speed is 3 frames per second.
