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Mitotic progression upon transient expression of double site rLIC1 phosphodeficient proteins in HeLa cells. Confocal fluorescence time-lapse videos of double stable GFP-α-tubulin (green)::histone 2B-mCherry (red) HeLa cells upon exogenous individual expression of rLIC1(S207E) constructs including double site rLIC1-CTD mutants SAA, ASA, and AAT. All videos show the duration from NEB to anaphase onset. Time stamps included. Scale bar = 10 µm. Speed is 3 frames per second.
