Extended Data Fig. 4: T cells in tumors with low neoantigen expression lose effector function over time.

(a-b) Percent of antigen-specific T cells from DLNs and tumors at 42 days negative for TCF1 and positive for TIM3 (a), and positive for TCF1 and negative for TIM3 (b) by flow cytometry. N = 4–5 independent animals per line. (c-d) Percent of antigen-specific T cells from DLNs and tumors double-positive for TNFα and IFNγ at 42 days (N = 4–5 independent animals per line) (c) and both 8 and 42 days (d) (N = 4–9 independent animals per line). Red = hi^SIIN, blue = lo^SIIN. Significance in (a-d) was assessed by Wilcoxon Rank Sum. Source data for panels (a-d) can be found in Source Data Figure 4d–e.