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. Author manuscript; available in PMC: 2021 Dec 8.
Published in final edited form as: Mucosal Immunol. 2021 Jun 8;14(5):1100–1112. doi: 10.1038/s41385-021-00419-1

Figure 8: Elevated IL-18 alters lamina propria immune cell composition in Rag1−/− and NLRC4TS/TS mice.

Figure 8:

Quantification of lamina propria lymphocytes by flow cytometry. Total live single CD45+ (a) and ILC (b) cell populations in Jackson WT vs Jackson and Pitt Rag1−/− mice. Representative flow plots and quantification showing KLRG1 NK1.1 double positive cells (c). T cell and NK cell composition (d and e) including representative flow plots and Tbet+ cells (f) in in house WT vs NLRC4TS/TS. Isolated ILCs were stimulated with Brefeldin, PMA and ionomycin for 2.5 hours and IFNg expression quantified (g). Panels a-c are representative on n=2 experiments with 3 mice per experimental group. Panels d-g show combined results from n=3 experiments with n=1–2 mice per group. Statistical significance is shown on each graph and was determined by unpaired t-test. Jax= Jackson Rag1−/− mice.