FIG. 3.

Deletion of JH2 enhances Jak2-mediated activation of Stat5. 293T cells were cotransfected with expression plasmids for Stat5 alone (—) or Stat5 plus Jak2-HA, ΔJH2-HA, JH1-2-HA, JH1-HA, or E665K-HA. Stat5 was immunoprecipitated with anti-Stat5 antibody, and the immunoprecipitates (IP) were analyzed by SDS–7.5% PAGE followed by antiphosphotyrosine (anti-PY) or anti-Stat5 immunoblotting. Aliquots of cell lysates were analyzed by SDS–4 to 15% PAGE followed by anti-HA immunoblotting (lowest panel). The mobilities of the molecular mass markers (in kilodaltons) are shown on the left.