Table 3.
Primer sequences and GenBank accession number for the target genes
| Target gene | Primer sequence (5′-3′) | Product length/bp | Acc. No.1) |
|---|---|---|---|
| GAPDH | PF: 5′-GCCATCACAGCCACACAGA-3′ PR: 5′-TTTCCCCACAGCCTTAGCA-3′ |
120 | NM_204305 |
| MSTN | PF: 5′-TTTTGGATGGGACTGGATTATAGCACCT-3′ PR: 5′-GCCTCTGGGATTTGCTTGGTGTACC-3′ |
127 | NM_001001461.1 |
PF, forward primer; FR, reverse primer; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MSTN, myostatin.
1)
Acc. No: GenBank accession number. Primers were designed according to the sequence of the target genes by there Acc. No. in GenBank.