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Time-lapse imaging of mitotic live bovine zygote expressing EB3-mEGFP2 (green) and H2B-mCherry (magenta) after mRNA injection at pronuclear stage. Time resolution; 2.5 min. Scale bar, 10 µm. Movie shows 30 frames/s. Recording starts at 2.5 min before synchronous NEBD. Movie shows spindle assembly in zygote depicted in Figs. 3 B and S4 D.
