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. 2021 Oct 8;220(11):e202102122. doi: 10.1083/jcb.202102122

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© 2021 Zhang et al.

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Figure S1. — RNAi efficiency and the effect of ACS family on LD size. (A and B) Fluorescence intensity (A) and quantification (B) of FAT-5::GFP{unc-119(ed3);kunIs161[fat-5p::fat-5::gfp,unc-119(+)]}, FAT-6::GFP {lin-15A(n765);waEx16[fat-6p::fat-6::gfp+lin15(+)]}, and FAT-7::GFP{lin-15(n765); waEx15[fat-7p::fat-7::gfp;lin-15(+)]}. Imaged by fluorescent microscopy (Olympus); scale bar represents 100 µm. The data are presented as mean ± SEM of 10 imaged worms. Significant difference between the control (EV) and a specific RNAi treatment: ***, P < 0.001. (C and D) Fluorescence intensity (C) and quantification (D) of ACS-1::GFP {unc-119(ed3);kunEx202[vha-6p::acs-1::gfp+unc-119(+), myo-2p::mcherry]}, and ACS-2::GFP {N2;[acs-2p::acs-2::gfp+rol-6(su1006)]} under acs-1RNAi treatment. The white arrows indicate the transgenic marker of MYO-2::mCherry (myo-2p::mcherry), and the purple triangle indicates ACS-1::GFP. Imaged by fluorescent microscopy (Olympus); scale bar represents 100 µm. The data are presented as mean ± SEM of 10 imaged worms. Significant difference between the control (EV) and a specific RNAi treatment: ***, P < 0.001. (E) Cluster analysis of ACS family in C. elegans and Homo sapiens. (F and H) LDs by Nile Red staining of fixed mutant worms (F) and RNAi-treated worms (H), cultured with E. coli OP50 and HT115, respectively. For all representative animals, posterior is left and anterior is right. Scale bar represents 5 µm. (G and I) The diameter (mean ± SEM) of LDs by quantification of five representative Nile Red–stained worms.