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. 2021 Oct 8;220(11):e202102122. doi: 10.1083/jcb.202102122

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© 2021 Zhang et al.

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Figure S4. — Separation of complex lipids by TLC and detection of C17iso in E. coli HT115 diet and C. elegans. (A–C) Separation of c-SM and c-Glc (A), c-Sph (B), and PL (c-PC, c-PE, c-PS, and c-PI; C) from TL by TLC analysis. (D) GC analysis indicates the presence (peak) of C17iso in E. coli HT115 diet (upper panels) and C. elegans N2 worms (lower panels) under different processing. The red arrows indicated the peak of C17iso. The black arrows indicate the flow direction of solvent. s-PC, s-PE, s-SM, and s-Sph are the standard lipids obtained from Sigma-Aldrich. c-TAG, c-PC, c-PE, c-SM, and c-Sph were isolated and separated from C. elegans wild type (N2).