Fig. 2.

Hamster GSCs are susceptible to Delta-24-RGD infection. (A) Viral replication in human, hamster, and mouse cells. 48 hours after infection with Delta-24-RGD (10 MOI), cells were lysed and the supernatant titered on A549 cells. Dashed line indicates input viral particles (1 × 10⁶ pfu/10⁵ cells). Data are shown as mean ± SEM, *adjusted P <.05, and ** adjusted P <.005. Dunnett’s test was used to compare each of the experimental arms with the control, adjusting for multiple comparisons. (B) Transmission electron microscopy 48 hours after virus infection demonstrates visible viral particles in human glioma cells (10 MOI) and hamster GSCs (100 MOI), but not in the mouse GL261 cell line (100 MOI). Scale bar = 500 nm. (C) IC₅₀ assays demonstrate that Delta-24-RGD kills human glioma cell lines and hamGSCs in a dose-dependent manner. (D) Representative H&E and immunohistochemistry for viral hexon protein in hamGSC-2 tumors 5 days after Delta-24-RGD treatment. Scale bars = 1 mm (1.25× objective), 200 µm (4× objective), and 100 µm (10× objective).