Table 2. Molecular features of the genes of H5N6 viruses isolated from humans.

Gene	GX01	GX11151	SC26221	GX31906	GZ39715	HB29578	Phenotypic characteristics
HA (H5 no.)
D94N	N	S	N	N	N	N	Increased virus binding to α2-6 SA
S133A	A	A	A	A	A	A	Increased pseudovirus binding to α2-6 SA
D155N	D	N	D	D	D	D	Increased virus binding to α2-6 SA
T156A	A	A	A	A	T	A
T188I	T	I	T	A	T	T	Increased pseudovirus binding to α2-6 SA
A263T	T	T	T	T	T	T	The residue is related to virulence.
222-224	QGG	QRG	QRG	QRG	QRG	QSG	222–224 QS(R)G avian-like α2–3 receptor binding preference
Cleavage peptides	RERRRKR	REKRRKR	REKRRKR	RERRRKR	RERRRKR	RERRRKR	Highly pathogenic avian influenza
NA (N6 no.)
E119D/V	E	E	E	E	E	E	Antiviral oseltamivir resistance
A247V	A	A	A	A	A	A
H274Y	H	H	H	H	H	H
R293K	R	R	R	R	R	R
R372K	R	R	R	R	R	R
PB2
K389R	R	R	R	R	R	K	Enhanced growth capacity in human and mammalian cells
V598T/I	T	T	T	T	T	V
PB1
I368V	I	I	I	I	I	V	Transmissible among ferrets
PA
N409S	S	S	S	S	S	N	Increased virus replicative ability in mammalian systems
M1
N30D	D	D	D	D	D	D	Increased virulence in mice
T139A	A	A	T	T	T	A
T215A	A	A	A	A	A	A
M2
D21G	G	D	D	D	D	G	Antiviral amantadine resistance
L26F/I	L	L	L	L	L	L
A30T	A	A	A	A	A	A
S31N	N	S	S	S	S	N
G34E	G	G	G	G	G	G
NS1
P42S	S	S	S	S	S	S	Increased virulence in mice
80-84Del	Yes	Yes	Yes	Yes	Yes	No
L98F	M	M	M	M	M	M