REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
BL21(DE3)	in house	N/A
DH10MultiBAC DH10EmBacY	in house	N/A
Chemicals, peptides, and recombinant proteins
Toyopearl AF-Chelate-650M	Tosoh	0014475
Flag M2 agarose beads	Sigma	A2220-25ML
Para-benzoyl phenylalanine	BACHEM	4017646.0001
Pierce Ultralink Streptavidin Resin	Thermo Scientific	53113
Culture Well Reusable Gaskets 3x1mm	Grace Biolabs/ Sigma	GBL103250
Hellmanex III	Hellma	9-307-011-4-507
Gamma-32-ATP	Hartmann Analytic	SCP-501
lambda DNA	New England Biolabs	N3011S
polyethylene imide	Sigma	1055790001
NADH	Sigma	N8129
Phosphoenolpyruvate	Sigma	N0564
lactate dehydrogenase/ pyruvate kinase	Sigma	P0294
avidin DN	Vectorlabs	A-3100-1
BSA (loop extrusion assays)	Thermo Scientific	AM2616
BSA fraction V (others)	VWR	422351S
ATP (loop extrusion assays)	Jena Biosciences	NU-1010
ATP (others)	Roche/ Sigma	ATPD-RO
Sytox Orange	Thermo Scientific	S11368
glucose oxidase	Sigma	G2133
Catalase	Sigma	C- 40
Trolox (loop extrusion assays)	Cayman Chemical	10011659
Trolox (smFRET)	Sigma	238813
Rapamycin	Sigma	R8781
glass rods	Hilgenberg GmbH	7001854
mica sheets	NanoAndMore GmbH	50-S-25-50-1
uhu two component epoxy	Conrad Electronics	478703
Cantilevers	Oxford Instruments	803.OLY.BL-AC10DS
sulfo Cyanine 3 maleimide	Lumiprobe	11380
sulfo Cyanine 5 maleimide	Lumiprobe	13380
Coenzyme A trilithium salt	Sigma	C3019
EZ-Link biotin-PEG11-maleimide	Thermo Scientific	21911
Dichloromethylsilane	Sigma	440272
Hexane	Sigma	139386
Sulfuric acid (for Piranha solution)	Sigma	258105
Hydrogen peroxide (for Prinanha solution)	Sigma	H1009
Permanent double sided sticking tape 3m	Scotch	N/A
Pluronic F-127	Sigma	P2443
Neutravidin	Thermo Scientific	31000
beta casein	Sigma	C6905
4-nitrobenzyl alcohol (NBA)	Sigma	N12821
Cyclooctotetraene (COT)	Sigma	138924
Protocatechuic acid (PCA)	Sigma	37580
Protocatechuic acid-3,4-Dioxygenase (PCD)	Sigma	P8279
2’,3’-O-Trinitrophenyl-adenosine-5’triphosphate	Jena Biosciences	NU-221
Platinum	Baltic	BP2261
Carbon	Balzers	B80100761
Cu/pd grids	Agar Scientific	AGG2440PD
Experimental models: Cell lines
SF9 insect cells	Thermo Scientific	B82501
Hela SCC1-Halo-Flag	Davidson et al. (2019)	N/A
Recombinant DNA
pET21a	Novagen	N/A
pEVOL-pBpf	Addgene	RRID:Addgene_31190
Plib	Weissman et al. (2016)	RRID:Addgene_80610
pBig1a	Weissman et al. (2016)	RRID:Addgene_80611
pBig1b	Weissman et al. (2016)	RRID:Addgene_80612
pBig1c	Weissman et al. (2016)	RRID:Addgene_80613
pBig2ab	Weissman et al. (2016)	RRID:Addgene_80616
Software and algorithms
Prism	Graphpad	Version 8
Fiji	NIH	Version 2.0.0
Gwyddion	http://gwyddion.net/	Version 2.55
Matlab	Mathworks	Version 2015b
iSMS	Preus et al. (2015)	Version 2.01
ebFRET	Van de Meent et al. (2014)	N/A
plotTDP Matlab script	http://ebfret.github.io/	N/A