Figure 5.

Peanut oral immunotherapy increases peptide-specific IgG levels while reducing abundance of pre-existing IgE specificities

(A) Change in seroprevalence of IgE (left) and IgG (right) against individual epitopes after OIT. Ara h 7 epitopes are highlighted in blue (IgE) and red (IgG). p values were calculated by Mann-Whitney test.

(B) Antibody binding Z score for a representative Ara h 7 epitope before (week 0) and after OIT (week 52). (Left) IgE Z scores are shown; (right) IgG Z scores are shown. Critical residues in the dominant footprint are highlighted in red. Each point represents one patient. Statistical significance was determined by paired t test.

(C) Change in IgE (blue) and IgG (red) binding Z score to pre-existing epitopes after OIT. Each bar represents the mean log2 fold change of Z scores for all pre-existing epitope peptides (Z score > 3.5 at week 0) at week 52 versus at week 0 for the OIT subject indicated by the x axis. Error bars denote SEM.

(D–F) Peptide binding Z scores changes of representative patients exhibiting both IgE decrease and IgG increase (D), IgE unchanged but IgG increase (E), and mixed changes in IgE and IgG (F). Only peptides with either week 0 or week 52 reactivity were examined. Each dot represents one peptide. Statistical significance was determined by Wilcoxon signed rank test. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant.