REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human IgE antibody, biotinylated	Southern Biotech	Cat#9160-08; RRID: AB_2796671
Anti-human IgG antibody, biotinylated	Southern Biotech	Cat#9042-08; RRID: AB_2796608
Biological samples
OIT allergic sera	MacGinnitie et al.17	Clinical trial NCT01781637, https://www.clinicaltrials.gov
Critical commercial assays
Human IgE ELISA kit	Bethyl	Cat#E80-108
Deposited data
Z-score data	This paper	Mendeley Data: https://doi.org/10.17632/zp97585dkp.1
Oligonucleotides
T7-PFA aatgatacggcggGAATTCCGCTGCGT	Xu et al.16	N/A
T7-PRA caagcagaagACTCGAGCTCTTCCCTG	Xu et al.16	N/A
Recombinant DNA
Peanut saturation mutagenesis AllerScan library	This paper	Annotations are available in Table S1 and are deposited at Mendeley Data: https://doi.org/10.17632/zp97585dkp.1
T7FNS2 vector	Larman et al.15	N/A
Software and algorithms
Bowtie	Langmead et al.37	http://bowtie-bio.sourceforge.net/index.shtml
Cutadapt	Martin38	https://cutadapt.readthedocs.io/en/stable/
R, version 3.6.1		N/A
GraphPad Prism version 9.1.2		N/A
Samtools version 1.3.1	Li et al.39	http://www.htslib.org
tidyverse	Wickham et al.40	CRAN: tidyverse; RRID: SCR_019186
Other
Pierce Streptavidin Magnetic Beads	Thermo Fisher Scientific	Cat#88817
T7 Select Packaging Kit	EMD Milipore	Cat#70014-3