Figure 2.

FILIP1L is downregulated in mucinous colon cancer. H&E staining (A, C, E, and G) and FILIP1L expression (B, D, F, and H) was analyzed in specimens from NATs (n = 16), serrated polyps (n = 9), well/moderately differentiated mucinous adenocarcinoma (n = 4), and poorly differentiated mucinous adenocarcinoma (n = 12). Note that multinucleated cells were not present in serrated polyps samples. Scale bar, 50 μm. I, FILIP1L expression in IHC stained slides (as shown in panels B, F, and H) was compared between matched normal (n = 16) and mucinous colon adenocarcinoma (n = 16). Expression score was carried out as described in Materials and Methods. J, FILIP1L mRNA expression was compared between normal (n = 5) and mucinous colon adenocarcinoma (n = 13). Data are derived from Oncomine public databases [Kaiser Colon (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE5206)]. K, NATs (n = 16), well/moderately differentiated nonmucinous colorectal adenocarcinoma (n = 9), and poorly differentiated nonmucinous colorectal adenocarcinoma (n = 7) were stained for FILIP1L, and expression score was carried out as described in I. L, DNA ploidy in 16 MAC tumors used in I was analyzed. FILIP1L expression score of each tumor is shown in table, and either diploid or aneuploid tumors were plotted against FILIP1L expression score. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.