Fig. 5.

SIV ddPCR viral quantitation in Rhesus macaque tissues at high nucleic acid input levels. ddPCR viral load measurement in DNA samples derived from up to 3.3 million cell equivalent of tissue samples (all from an SIV-infected and ART suppressed animal (#27882)) in each reaction. Reactions were performed in triplicates when sample quantity allowed. “SIV+” and “SIV + CCR5+” indicate SIV positive and SIV & CCR5 double positive droplets, respectively. qPCR viral load data in the same samples were obtained using an approach where the DNA samples were diluted, and tested in up to 10 replicate reactions per sample, and quantitative viral load results derived either from SIV DNA standard curve (when all 10 reactions were PCR positive) or based on Possion statistics (when some of the reactions were PCR negative), as described in [55], [56]. Note that the liver DNA samples (B-D) were from 3 different tissue sections (LM2, RL2x1 and LM2x1, respectively). Samples details and quantitation results are summarized in (G).