Table 1.

Sequences of primers and probes used in the reverse transcription and ddPCR steps.

Primer	Sequence	Note
SIVNestR01	GTTGGTCTACTTGTTTTTGGCATAGTTTC
SGag forward	GTCTGCGTCAT(dP)TGGTGCATTC	dP [57] denotes a nonstandard base
SGag reverse	CACTAG(dK)TGTCTCTGCACTAT(dP)TGTTTTG	dK [57] and dP denote nonstandard bases
RCCR5 forward	CCAGAAGAGCTGCGACATCC
RCCR5 reverse	GTTAAGGCTTTTACTCATCTCAGAAGCTAAC
Probe	Sequence
SGag ddPCR probe	5′-FAM- CTT CYT CAG TRT GTT TCA CTT T -MGB
RCCR5 ddPCR probe	5′ VIC- TTC CCC TAC AAG AAA CT-MGB