Table 1.
Sequences of primers and probes used in the reverse transcription and ddPCR steps.
| Primer | Sequence | Note |
|---|---|---|
| SIVNestR01 | GTTGGTCTACTTGTTTTTGGCATAGTTTC | |
| SGag forward | GTCTGCGTCAT(dP)TGGTGCATTC | dP [57] denotes a nonstandard base |
| SGag reverse | CACTAG(dK)TGTCTCTGCACTAT(dP)TGTTTTG | dK [57] and dP denote nonstandard bases |
| RCCR5 forward | CCAGAAGAGCTGCGACATCC | |
| RCCR5 reverse | GTTAAGGCTTTTACTCATCTCAGAAGCTAAC | |
| Probe | Sequence | |
| SGag ddPCR probe | 5′-FAM- CTT CYT CAG TRT GTT TCA CTT T -MGB | |
| RCCR5 ddPCR probe | 5′ VIC- TTC CCC TAC AAG AAA CT-MGB |