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. 2021 Oct 20;12:754005. doi: 10.3389/fphar.2021.754005

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Copyright © 2021 Yu, Hong, Liu, Wu, Xie, Fang and Zhi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cordycepin reduced mitochondria-dependent intrinsic apoptosis in HG/HF cultured SI/R cardiomyocytes. (A) Flow cytometry of apoptosis by annexin V and propidium iodide (PI) double staining (left) and quantification of apoptotic cells (right) in cardiomyocytes with treatment as indicated. (B, C) Western blotting and quantitative analysis of cytosolic cytochrome C release, cleaved caspase 9 levels and cleaved caspase 3 levels in cardiomyocytes. HG/HF, high-glucose/high-fat; SI/R, simulated ischemia/reperfusion; Cor, cordycepin. **p < 0.01. n = 3–4 wells.