Figure 1.

Expression patterns of the CaPRR2 gene. (A) Phylogenetic tree analysis of the CaPRR2 protein and its homologous proteins in Arabidopsis thaliana. Using a BLAST search with CaPRR2 as query, protein sequences of A. thaliana were retrieved. For multiple sequence alignment, the web tool Clustal Omega³ was used with the default settings. The phylogenetic tree was generated using MEGA X software on the basis of multiple alignments of CaPRR2 and its homologous proteins from A. thaliana in ClustalW2. (B) Tissue-specific expression of CaPRR2 in various pepper plant tissues at the seedling and mature stages. YL, young leaf; RT, root; ST, stem; CT, cotyledon; FEL, fully expanded leaf; PT, petiole; TR, tap root; LR, lateral root; FL, flower; FRT, fruit. (C) Expression patterns of CaPRR2 in the leaves of pepper plants exposed to abscisic acid (ABA; 100μM), drought, H₂O₂ (100mM), mannitol (600mM), NaCl (200mM), or low temperature (10°C). The pepper Actin1 (CaACT1) gene was used as an internal control. Data represent the mean±SE of three independent experiments; asterisks indicate significant difference compared with the untreated control (0h; Student’s test; p<0.05).