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. 2021 Oct 20;12:736421. doi: 10.3389/fpls.2021.736421

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Copyright © 2021 Lim, Lim and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enhanced drought tolerance of CaPRR2-silenced pepper plants. (A) Drought sensitivity of CaPRR2-silenced (TRV2:CaPRR2) and control (TRV2:00) pepper plants. Three-week-old plants of each line were exposed to drought stress by withholding watering for 14days. After re-watering for 2days, the representative images were taken. (B) Survival rates of pepper plants after re-watering. Data represent the mean±SE of three biological replicates, each evaluating 20 plants. (C) Relative water contents of TRV2:CaPRR2 and TRV2:00 plants exposed to drought stress. (D) Transpirational water loss from the leaves of TRV2:CaPRR2 and TRV2:00 plants. Leaves were detached and the fresh weights of each line were measured at the indicated time points. (E,F) Enhanced leaf temperatures of CaPRR2-silenced (TRV2:CaPRR2) and control (TRV2:00) pepper plants in response to abscisic acid (ABA) treatment. Thermal images of CaPRR2-silenced and control pepper plants before and after ABA treatment (E). Average leaf temperatures of CaPRR2-silenced and control pepper plants before and after ABA treatment (F). (G,H) Stomatal apertures of CaPRR2-silenced (TRV2:CaPRR2) and control (TRV2:00) pepper plants in first and second leaf peels incubated in stomatal opening solution containing 0μM or 20μM ABA. Stomatal pore images of CaPRR2-silenced and control pepper plants. (G) Stomatal apertures (width/length) of CaPRR2-silenced and control pepper plants (H).