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. 2021 May 20;28(11):3077–3091. doi: 10.1038/s41418-021-00803-1

Fig. 1. TRIM26 positively regulates TLRs-induced production of proinflammatory cytokines.

Fig. 1

A qPCR analysis Tnfα, Il6, and Il12b mRNA expression in PMs prepared from Trim26+/+ and Trim26–/– mice stimulated with LPS (200 ng/ml) for the indicated times. B ELISA of TNF-α, IL-6, and IL-12p40 protein in supernatant of PMs prepared from Trim26+/+ and Trim26–/– mice stimulated with LPS for 12 h. C qPCR analysis of Tnfα, Il6, and Il12b mRNA, respectively, in PMs prepared from Trim26+/+ and Trim26–/– mice, stimulated with Pam3CSK4 (1 μg/ml), Poly(I:C) (20 μg/ml), and R848 (10 μg/ml) for 6 h. D ELISA analysis of TNF-α, IL-6, and IL-12P40 protein, respectively, in PMs prepared from Trim26+/+ and Trim26–/– mice, stimulated with Pam3CSK4 (1 μg/ml), Poly(I:C) (20 μg/ml), and R848 (10 μg/ml) for 12 h. E qPCR analysis of Tnfα, Il6, and Il12b mRNA expression in BMDMs prepared from Trim26+/+ and Trim26–/– mice, stimulated with LPS (200 ng/ml) for the indicated times. F qPCR analysis of Tnfα, Il6, and Il12b mRNA in BMDMs prepared from Trim26+/+ and Trim26–/– mice, stimulated with Poly(I:C) (20 μg/ml) and R848 (10 μg/ml) for 6 h. G qPCR analysis of Tnfα and Il6 mRNA expression in MEFs prepared from Trim26+/+ and Trim26–/– embryos (day 13.5), stimulated with LPS (200 ng/ml) for the indicated times. H qPCR analysis of Tnfα and Il6 mRNA, respectively, in MEFs prepared from Trim26+/+ and Trim26–/– embryos (day 13.5), stimulated with Pam3CSK4 (1 μg/ml), Poly(I:C) (20 μg/ml), and R848 (10 μg/ml) for 6 h. Data are shown as mean ± SD of triplicates from one representative experiment in A–H. *p < 0.05, **p < 0.01; Student’s t test. Similar results were obtained in three independent experiments.