Skip to main content
. 2021 Feb 8;42(11):1821–1833. doi: 10.1038/s41401-020-00601-4

Fig. 1. Berberine prevented stress-induced visceral hypersensitivity and microglial activation.

Fig. 1

a Design of the WAS and berberine treatment experiments. n = 10/group. b Fecal pellet output number in the container after each 1-h WAS. n = 10/group. c Abdominal withdrawal reflex scores in response to CRD. n = 10/group. d Levels of serum CRH. n = 7/group. e Number of tryptase-positive cells in the distal colonic mucosal tissues. f Immunohistochemistry showing tryptase-positive cells in the distal colon. n = 7/group. g Representative images of Iba-1-stained microglia in the dorsal lumbar spinal cord. h Number of Iba-1-positive cells in the dorsal lumbar spinal cord. i Representative three-dimensional reconstructions of Iba-1-stained microglia residing in the dorsal lumbar spinal cord. j Morphological parameters of microglia. n = 3/group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.