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. 2021 Nov 2;11:21466. doi: 10.1038/s41598-021-00908-0

Table 1.

RT-qPCR primer sequences in this study.

Gene name Gene bank number Sequence(5′–3′) Tm (℃) Product size (bp)
ACTB NM_001101.5 Forward TGGCACCCAGCACAATGAA 69.0 186
Reverse CTAAGTCATAGTCCGCCTAGAAGCA 65.4
BAX NM_138761.4 Forward CTCAGGATGCGTCCACCAA 67.5 83
Reverse CCTCTGCAGCTCCATGTTACTGTC 68.1
BCL2 NM_000633.2 Forward AACATCGCCCTGTGGATGAC 67.3 146
Reverse AGAGTCTTCAGAGACAGCCAGGAG 66.3
HSPA1A NM_005345.6 Forward CCTGGAGTCCTACGCTTCAAC 68.0 104
Reverse CTTGACACTTGTCCAGCACCTTC 67.1