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. 2021 Nov 2;11:21533. doi: 10.1038/s41598-021-00958-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EGFP-TOP2B is highly mobile in the nucleoplasm and nucleolus. Experiments were performed on EGFP-TOP2B as described in Fig. 3. (a, b) Representative images of FRAP analysis of EGFP-TOP2B in the nucleoplasm (a) and in the nucleolus (b). (c, d) Quantification of fluorescence signals of EGFP-TOP2B in the nucleoplasm (c) and in the nucleolus (d).