TABLE 3.
Strain (allele) | Recombination frequency at:
|
|||||
---|---|---|---|---|---|---|
his4
|
leu2
|
|||||
0 h | 24 h | Fold increase | 0 h | 24 h | Fold increase | |
CJY1 (wild type) | 9.3 × 10−4 | 9.6 × 10−2 | 103.2 | 2.0 × 10−5 | 5.7 × 10−3 | 285 |
CJY2 (mei4) | 7.9 × 10−4 | 9.0 × 10−4 | 1.1 | 1.6 × 10−5 | 2.0 × 10−5 | 1.3 |
CJY11 (rad50) | 5.9 × 10−4 | 8.5 × 10−4 | 1.4 | 3.7 × 10−4 | 3.1 × 10−4 | 0.8 |
CJY15 (rec102) | 1.2 × 10−3 | 1.1 × 10−3 | 0.9 | 1.4 × 10−5 | 1.0 × 10−5 | 0.7 |
CJY19 (rec104) | 7.7 × 10−4 | 8.4 × 10−4 | 1.1 | 1.7 × 10−5 | 1.7 × 10−5 | 1 |
CJY30 (rec114) | 2.2 × 10−3 | 3.3 × 10−3 | 1.5 | 1.7 × 10−5 | 2.2 × 10−5 | 1.3 |
CJY25 (xrs2) | 5.3 × 10−4 | 5.8 × 10−4 | 1.1 | NDb | ND | |
CJY24 (mre11) | 1.4 × 10−3 | 3.7 × 10−3 | 2.6 | 4.0 × 10−4 | 8.8 × 10−4 | 2.2 |
CJY57 (mer2) | 1.0 × 10−3 | 9.0 × 10−4 | 0.9 | 2.1 × 10−5 | 1.3 × 10−5 | 0.6 |
CJY29 (spo11) | 6.4 × 10−4 | 9.6 × 10−4 | 1.5 | 3.6 × 10−5 | 4.0 × 10−5 | 1.1 |
Sporulation was carried out as described in Materials and Methods. The data presented are averages of three independent experiments for each mutant strain.
ND, not determined.