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. 2021 Nov 2;11:21439. doi: 10.1038/s41598-021-00863-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HL-60 cells differentiated to neutrophil-like cells by all–trans-retinoic acid (ATRA). (A,B) Growth curve. HL60 cells (5 × 10⁵/mL) were seeded into a T-75 flask and treated with 0 or 1 μM ATRA once every 2 to 3 days for 5 days. Cells not stained by trypan blue were counted for 9 days. (C) Nuclear morphology of HL60 cells. HL60 cells were treated with 0 or 1 μM ATRA for 6 days and stained with Wright-Giemsa. At day 0, the nuclear morphology of dHL60 cells was ovate (left) but showed a dent (center) or lobular shape at 6 days (right) (magnification, ×400). (D,E) FACS analysis of CD11b expression in dHL60 cells. HL60 cells were differentiated by 0 or 1 μM ATRA for 6 days and stained with an anti-CD11b-FITC antibody. IgG was used as the negative control. Expression of CD11b (left). Percentage of total cells expressing CD11b (right). Independent sample t-test was used to find the difference.