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. 2021 Nov 2;11:21534. doi: 10.1038/s41598-021-00966-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mutations in SlINVINH1 of the genome edited lines. Target sequence in original cultivar ‘Suzukoma’ is shown at the top in each panel and the sequence in three genome edited lines (193–3, 199–2, and 247–2) are shown below. Grey shade indicates PAM sequence. Red shade, blue shade or red small letter show insertion, deletion, or substitution of nucleotide(s), respectively.