Figure 4 .

Characterization of genome-wide PARIS binding sites in SH-SY5Y neuroblastoma cells. (a) Human neuroblastoma cells provide a potential niche for PPARγ’s regulatory activities, justifying the choice of cell line for ChIP-seq experiment. (b) An upset plot¹⁹ showing the majority of significant PARIS ChIP-seq peaks overlap promoter region (− 3000, + 3000) of the genes in the human genome. (c) Enrichment of neuron-specific cellular components by PARIS taking the entire set of the peak-annotated genes as input. (d) Enriched metabolic pathways identified by BioCarta MetabolicPA module in EnrichR²⁷ taking as input the significant peak-annotated genes that are also ranked by fold enrichment and that have a ChIP-seq peak overlapping the promoter region. Top 3 pathways include PPARγ and NRF2 pathways. (e) Enriched TFs identified by Transcription Factor PPI module in EnrichR²⁷ taking the same genes in (d) as input. The most enriched TFs include PPARẟ and RXRA, the binding partner of PPARγ.