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. 2021 Nov 3;6(1):25. doi: 10.1186/s41231-021-00101-5

Fig. 3.

Fig. 3

Representative immunofluorescence images of PB-NHESC-C. Legend: Sample of the PB-NHESC-C were stained with FITC-conjugated monoclonal surface antibody CD45 (1:100) and Hoechst nucleic acid dye 33,342 (10 μg/ml). Images were acquired using a Leica SP8 confocal microscope using a 63x objective. Two main subpopulations were identified: the Non-hematopoietic (*) and the Hematopoietic Stem Cells (arrow)