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. 2021 Nov 2;11:21517. doi: 10.1038/s41598-021-01082-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design. (A) Group 1 animals underwent bilateral ovariohysterectomy (OSH). Hemi-ovaries were cryopreserved and stored in liquid nitrogen (− 196 °C) for seven days. (B) Seven days after cryopreservation, hemi-ovaries were thawed and transplanted to Group 2 animals, forming the Cryopreserved transplanted group. Group 2 animals underwent OSH in the same surgical procedure, with the hemi-ovaries immediately transplanted to Group 3 animals, constituting the Fresh transplanted group. Hemi-ovaries from Group 3 animals were obtained by OSH, cryopreserved and thawed after 7 days, but not transplanted (Cryopreserved non-transplanted group). Group 4 animals were euthanized, and the hemi-ovaries obtained immediately evaluated as a fresh tissue (Fresh non-transplanted group). Seven days after hemi-ovary transplantation to Groups 2 and 3, animals were euthanized, and hemi-ovaries retrieved for analysis. Hemi-ovary samples from all groups, were distributed between the analysis, with half of the hemi-ovaries fixed for histological analysis, and the other used to evaluate mitochondrial oxygen consumption. OSH = ovariohysterectomy; OT = ovarian tissue, corresponding to a hemi-ovary. This figure was created by the authors using Biorender (https://biorender.com/).