FIG. 1.

Phenotypes of the eso1-H17 mutant. (A) The eso1-H17 mutant shows an elongated cell morphology. Wild-type (h⁻ leu1-32) and eso1-H17 (h⁻ eso1-H17 leu1-32) cells were inoculated on YEA plates, incubated overnight at the indicated temperatures, and observed under the microscope. (B) The eso1-H17 mutant is arrested with a broad peak of G₂ DNA content. Cells were grown to mid-log phase at 23°C in MM medium and shifted up to 34°C. Cells were sampled at 2, 4, 6, and 8 h after the temperature shift and analyzed by flow cytometry.