FIG. 7.

Phenotypes of eso1 null mutants suppressed by amino-terminally truncated eso1⁺. (A) Nuclear morphologies of Δeso1 cells (h⁻ ade6-M210 eso1::ura4⁺ ura4-D18 leu1-32) suppressed by full-length eso1⁺ (pcL-full) or its amino-terminal truncation mutants (pcL-ΔN458 and pcL-ΔN597). Cells were grown to mid-log phase at 30°C in YEL medium, fixed with glutaraldehyde, and stained with DAPI. (B) Bleomycin and UV sensitivities. Approximately 5 × 10⁴, 5 × 10³, 5 × 10², and 50 cells of the wild type (h⁻ ade6-M210) and the Δeso1 mutant (h⁻ ade6-M210 eso1::ura4⁺ ura4-D18 leu1-32) suppressed by pcL-full, pcL-ΔN458, and pcL-ΔN597 were spotted on normal YEA plates (referred to as control), YEA plates containing bleomycin (0.02% [wt/vol]), or normal YEA plates but with irradiation with UV (100 J/m²). Plates were incubated at 30°C for 3 days. (C) Growth curves. Δeso1 cells (h⁻ ade6-M210 eso1::ura4⁺ ura4-D18 leu1-32) suppressed by pcL-full, pcL-ΔN458, or pcL-ΔN597 were grown at 30°C in MM plus adenine. (D) UV sensitivity. Δeso1 cells (h⁻ ade6-M210 eso1::ura4⁺ ura4-D18 leu1-32) suppressed by pcL-full, pcL-ΔN458, or pcL-ΔN597 were plated on YEA, irradiated with various doses of UV, and incubated for 5 days at 30°C.