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Schematic representation of the results. Cardiomyocyte differentiation at an initial high cell density (a) and at an initial low cell density (b). Mesodermal cells (gray round) induced by Wnt activators secrete auto/paracrine factors that may suppress Wnt signaling. At an initial low cell density, the suppression provided by the auto/paracrine factor is weak. Early addition of Wnt signaling inhibitors suppresses including non-cardiac mesoderm genes to induce cardiomyocytes.
