Fig. 2.

Comparison of PhaCCA and GstCCA thermal stability by NanoDSF. A) Representative curves are shown for the two apo enzymes with the variation of fluorescence at 330 and 360 nm, as well as the scattering signal, whose increase confirms protein unfolding. Melting temperatures (Tm) correspond to the inflection point of the first derivatives. B) Tm values in the absence and presence of nucleotides, and associated standard deviations calculated as the mean of three values (Tm from 330 nm, 350 nm fluorescence and from scattering) for at least 3 independent samples.