FIG. 9.

Tax promotes association of CBP with GAL4-CREB-2 bZIP in vivo. CEM cells were cotransfected with 2 μg of the luciferase reporter vector pG5luc, 5 μg of pACβ1, 2.5 μg of a eukaryotic vector expressing a GAL4-CREB-2 bZIP fusion protein, 1 μg of a plasmid expressing the WT Tax or the Tax point mutant K88A, and 10 μg of pRSV-CBP. The total amount of DNA in each series of transfections was the same, the balance being made up with the empty plasmids. Luciferase values were normalized for β-galactosidase activity and are expressed as fold increase relative to that of cells transfected with pSG-5, pCI-neo, pG5luc, and the eukaryotic vector expressing GAL4-CREB-2 bZIP. Values are the means ± standard deviations (n = 3).